3D culture techniques have contributed to the development of organoids, opening the door to a wide field of applications solving part of the limitations of monolayer cultures. They represent the cellular, structural and functional heterogeneity of tissues in vivo, being very useful in the study of therapeutic targets or in drug screening.

The Biobank of the Aragon Health System (BSSA), in coordination with the Spanish Biomodel Platform, has among its objectives the creation of a collection of organoids representative of different human pathologies, to make them available to the scientific community. Firstly, we decided to create a collection of matched tumor and healthy organoids from different tissues. To this end, the following specific methods were established: the generation, maintenance and characterization by Hematoxylin/Eosin staining, immunohistochemistry and RT-PCR of colon, kidney, lung, gliobastoma and breast organoids from donor fresh tissue and their storage and subsequent thawing. Also we have done Cell Titter Glo experiments in order to study the toxicity of different compounds. This work has made possible to obtain and characterize different organoids from healthy or tumor tissues, which has led to the start of a collection of organoids within the BSSA and their transfer to various projects.