Osteochondral tissue regeneration is a significant challenge in regenerative medicine. Despite the many existing techniques using different types of biomaterials and biomolecules, a fully functional cure has not yet been found because of the complexity to mimic the multiphasic properties of this tissue. Decellularised extracellular matrix (dECM)-based bioinks as part of osteochondral grafts aims to fill these gaps by preserving and maintaining the structural and biochemical interactions of the native environment. In this study, cartilage-dECM and bone-dECM were obtained from pre-differentiated human mesenchymal stem cells (hMSC) under a combination of different factors to generate bioinks for cartilage and bone repair. Cartilage-dECM and bone-dECM were characterised and evaluated in vitro by histological and immunofluorescence techniques assays. The addition of these dECMs to a combination of fluid biomaterials allowed the generation of novel 3D bioprintable bioinks that were subsequently evaluated by assessing cell viability, biocompatibility and cell differentiation by immunofluorescence staining assays. The results showed that cartilage-dECM and bone-dECM possess excellent biochemical properties for use in combination with other biomaterials for osteochondral regenerative medicine. In addition, hydrogels generated embedded with hMSC using dECM-based bioinks were shown to maintain cell viability and proliferation over time and appear to induce differentiation after 21 days in standard cell culture conditions. In conclusion, our results indicate that dECM-based bioinks are biocompatible, easily adaptable, and low-cost biomaterials and that cartilage-dECM and bone-dECM 3D-bioprintable bioinks could be successfully applied as implants to promote regeneration in osteochondral damaged tissue.