Naive human pluripotent stem cells are utilized as a model system for developmental processes as they are defined as the in vitro equivalent of the human preimplantation embryo's epiblast. In our investigation, we have uncovered and characterized diverse cell populations existing alongside epiblast-like cells in human naive induced pluripotent stem cells (hiPSCs) cultures in 5iLAF medium. These cell populations bear a striking resemblance to various cell types observed in the human embryo during early developmental stages. Among these, the dominant cell population are epiblast-like cells of the human embryo, however, we have also identified a cell population expressing genes and transposable elements that closely resemble the eight-cell stage (8C) totipotent human embryo. We have also described for the first time three cell populations similar to trophectodermal (TE) cells at different stages of their maturation process: transition, early, and mature TE. Furthermore, in these cultures we have also found cells resembling primitive endoderm (PrE). Therefore, 5iLAF naive hiPSC cultures provide an excellent opportunity to study the earliest events of human embryogenesis, from the 8C stage to the peri-implantation period.