Stress urinary incontinence (SUI) is a highly prevalent chronic disease, associated with population ageing and other risk factors. Current treatments are directed against the symptoms and do not effectively restore sphincter function. Complications associated with some of the treatments, in particular synthetic slings, are of growing concern to patients and authorities. We aimed to generate a 3D-printed cell wrap as a treatment for SUI that induces regeneration of the rhabdosphincter (skeletal muscle ring), thus providing contractile properties to the urinary sphincter. First, we designed a tissue-specific protocol for porcine muscle decellularisation that ensured the maintenance of a significant proportion of muscle extracellular matrix proteins, while effectively removing any cellular remnants. Decellularised muscle tissue was processed to obtain a bioink, which was further complemented with other biomaterials to improve its rheological properties. The resulting bioink proved to be biocompatible and permit cell adhesion. For the cellular portion of the bioinks, mouse myogenic precursors were used. Myosphere cultures were derived from disaggregated mouse muscle cells, subjected to cell sorting based on the CD56 marker. The myogenic potential of CD56⁺ cell fraction was evaluated in comparison with the CD56- cell fraction and the total cell pool. The CD56⁺ population showed overexpression of the satellite cell marker Pax7, together with myogenic markers MYOG, MYOD and MYF5. In addition, this population resulted in a culture with increased myotube maturation. We are currently  evaluating muscle formation of CD56⁺ cells on the porcine dECM-based muscle bioink to determine is suitability as a potential SUI treatment.