The use of decellularized extracellular matrix (dECM) as a biomaterial has been a significant breakthrough in the field of regenerative medicine, as it provides a promising approach to generate functional tissue substitutes. By removing cellular components, dECM retains the native architecture and biochemical properties of the original tissue, providing an ideal scaffold for tissue engineering. Moreover, using patient-derived primary cultures as a source of ECM is a highly personalized approach that allows for the recreation of the structure, components, and functionality of the native context. In this project, a range of bioinks based on dECM was developed from mesenchymal stem cells (MSCs), fibroblasts (FBs), and MSCs that have been differentiated into adipocytes. These bioinks were utilized to replicate the primary layers of skin tissue in tissue substitutes, providing a highly personalized approach that can be tailored to the specific needs of the patient. The use of patient-specific dECM bioinks and 3D bioprinting technology has significant potential for the development of highly functional tissue substitutes that can closely replicate the structure, composition, and function of the native tissue.