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Wound healing gene expression in a pressure ulcer animal model

S Perez-Lopez(1) M Perez-Basterrechea(1) J M Garcia-Gala(1) M A Fernandez-Rodriguez(1) E Martinez-Revuelta(1) M Alvarez-Viejo(1)

1:Cell Therapy and Regenerative Medicine Unit. Hematology and Hemotherapy Department. Central University Hospital of Asturias (HUCA). Health Research Institute of Asturias (ISPA). Oviedo. 33011. Spain.

Hard-to-heal wounds are complex injuries with tendency to become chronic, between them, Pressure Ulcers (PUs) are ischemic injuries affecting skin and underlying tissue as result of intense and/or prolonged pressure, alone or in combination with shear and/or friction. PUs also has expensive time-consuming treatments and long-term hospitalizations. Advanced Therapies seems to be an interesting therapeutic approach to achieve a healing alternative treatment. In order to perform procedures those for methodological and/or ethical reasons are not viable in the clinical practice and to improve knowledge of this kind of lesions, pre-clinical pressure ulcer Swiss nu/nu mice model was developed using neodynium magnets. Afterwards, in order to get healing, the generated lesions were treated with a blood plasma-based scaffold combined or not with bone-marrow mononuclear cells isolated from luminescent strain FVB/N-Tg (β-Actin-luc)-Xen mice. Animals have been randomly assigned to three experimental groups (Control, Scaffold and Scaffold combined with cells). Lesion tissue biopsies were obtained after 3 and 7 days of treatment in order to investigate molecular response in the wound. The total RNA was isolated and each sample was reverse transcribed to complementary DNA. A wound-healing specific array (Wound Healing RT2 Profiler PCR Array, QIAGEN) was used for the molecular study of gene expression. Genes related with extracellular matrix, adhesion molecules, inflammatory cytokines, growth factors and transduction signals were included. After data processing using GeneGlobe software, it could be observed differences in terms of gene expression between experimental groups, being significant in some cases. Obtained results will be discussed in the poster.

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