P59

Mineralized Eggshell Membranes as an Osteoinductive Biomaterial for Bone Regenerative Medicine

A Voltes-Martínez(1) A Torres-Mansilla(2) P Álvarez-Lloret(2) J Gómez-Morales(3) J A Marchal(1) E López-Ruiz(4)

1:Universidad de Granada; 2:Universidad de Oviedo; 3:Instituto Andaluz de Ciencias de la Tierra (IACT-CSIC); 4:Universidad de Jaén

Regeneration of damaged bone tissue focuses on the attempt to repair bone lesions that the body fails to heal using different combinations of synthetic and natural biomaterials. Hybrid biomimetic materials aim to replicate the organic-inorganic interactions of mineralized biological tissues. Eggshell membrane (ESM) can control the nucleation of calcite crystals during eggshell formation, constituting a potential organic template for the controlled precipitation of other mineral phases. Different biomimetic strategies have been studied to improve the properties of synthetic nanocrystalline apatites using a vapor diffusion seated drop (VDSD) crystallization methodology. This approach favors the microenvironment for the controlled precipitation of calcium phosphate (CaP), an essential mineral in bone tissue. In this study, a biological organic surface, such as eggshell membranes (ESMs), has been used to control the microstructural properties during the mineralization process. The results show that ESMs mineralized by VDSD give rise to a CaP/ESM biomimetic material that is similar in composition and properties to mineralized tissues. Moreover, in-vitro studies revealed that it is biocompatible and favors the osteogenic differentiation of hMSCs after 21 days in culture. We conclude that this new natural biomaterial could be developed for guided bone regeneration and restorative dentistry. 

This research was supported by the Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (FEDER funds, DTS21/00098), Fundación Mutua Madrileña (FMM-AP17196-2019  and by the Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía (PYC20.RE.015.UGR).