1:Pall Life Sciences

Clarification with Pall Seitz® Depth Filters for Adherent and Suspension Adeno-Associated Virus Culture

Sarah Blackmore, Sabrina Cencig and Heather Griffith

Pall Biotech, 20 Walkup Dr, Westborough, MA, USA; Pall BVBA, 2 Reugelstraat, 3320 Hoegaarden, Belgium 

In recent years, we have seen a rapid increase in the gene therapy market and the number of new therapies using adeno-associated viruses (AAV) as a viral vector. Our objective was to determine the most efficient filter trains for the scalable clarification of both adherent-based and suspension-based AAV cultures.

Based on the analysis of data from >300 global studies, we examined throughput, recovery, flux and turbidity differences across multiple serotypes. The screening of Seitz filters for the AAV harvest resulted in a choice depending on two main parameters: the type of culture (adherent versus suspension) and the specific cell culture properties (including initial turbidity of the harvest, lysis and benzonase treatment).

Based on this analysis, for suspension AAV culture, we recommend evaluating the PDP8 filter in combination with the V100 or BIO20 filter. For adherent AAV culture, we recommend testing the PDK11 filter, a combination of PDP8 & V100 filter or the single layer V100 only for a very low turbidity harvest.